日糧VA影響斷奶仔豬腸道干細胞，進而影響生長性能、腸道發(fā)育和功能

發(fā)布單位:天津瑞孚農(nóng)牧科技集團有限公司

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時間：2020-03-24
       VA是斷奶仔豬的關鍵營養(yǎng)之一，它在維持上皮細胞正常形態(tài)、發(fā)育上起著重要作用。前人的研究表明添加VA可以改善機體腸道屏障功能。因此，我們假設日糧中添加VA可以通過調(diào)節(jié)腸道干細胞影響腸道功能。

       試驗選用32頭21d斷奶仔豬（（約克夏×長白）×杜洛克），平均重8.34±0.13kg，隨機分至4個處理組，VA水平分別為2（對照組）、4、8、16mg/kg。試驗期14d。斷奶仔豬自由采食和飲水。

       試驗第8～14d仔豬日增重和肉料比隨VA添加水平的升高而線性提高（P=0.02、P=0.005）。16mg/kg VA組空腸Lgr5+基因表達顯著提高（P=0.012）。4 mg/kg VA組的空腸絨毛高度和絨毛表面積顯著提高（P值分別為0.027和0.035）。4、8mg/kg VA組隱窩深度顯著增加（二次方程式，P=0.043），16mg/kg VA組絨毛高度：隱窩深度顯著提高（二次方程式，P=0.015）。當進一步補充4mg/kg VA時，麥芽糖酶、蔗糖酶和堿性磷酸酶活性顯著提高（P=0.032、0.041、0.024）。2mg/kg VA組的Slc2a2 mRNA豐度顯著提高（線性，P=0.024）。此外，VA及其代謝產(chǎn)物（視黃酸）顯著降低了仔豬腸道類器官的出芽率，每個類器官的出芽數(shù)以及嗜鉻粒蛋白A和Muc2的表達（P <0.05）。與對照組相比，Spp1和Trop2的表達顯著提高。

      這些結果表明VA可能會增加體外腸道干細胞的干性。本試驗結果表明VA能通過調(diào)節(jié)空腸腸道干細胞影響斷奶仔豬生長性能和腸道功能。

Dietary vitamin A affects growth performance, intestinal development, and functions in weaned piglets by affecting intestinal stem cells

Vitamin A (VA) is an important nutrient for weaning piglets. It plays a significant role in the normal formation, development, and maintenance of epithelial cells. Previous studies have shown that VA supplements could improve the host’s intestinal barrier function. Therefore, we hypothesized that VA supplements can affect intestinal function in weaned piglets by regulating intestinal stem cells. Thirty-two 21-d-old weaned [(Yorkshire × Landrace) × Duroc] piglets with an average weight of 8.34 ± 0.13 kg were randomly divided into 4 treatment groups, with 1) 2 mg/kg (control), 2) 4 mg/kg, 3) 8 mg/kg, and 4) 16 mg/kg doses of VA, respectively. The experiment lasted for 14 d. Weaned piglets were given ad libitum access to food and water during the test. The ADG (linear, P = 0.020) and G:F (linear, P = 0.005) of the piglets were found to increase significantly from days 8 to 14. The Lgr5+ gene expression (P = 0.012) in the jejunum mucosa of the 16 mg/kg VA group was increased. The jejunum villus height (P = 0.027) and villi surface area (P = 0.035) were significantly increased in the 4 mg/kg VA treatment group. The crypt depth increased significantly in the 4 and 8 mg/kg VA treatment groups (quadratic, P = 0.043), and the ratios of villus height to crypt depth significantly increased in the 16 mg/kg VA group (quadratic, P = 0.015). The maltase (P = 0.032), sucrose (P = 0.041), and alkaline phosphatase activity (linear, P = 0.024) were significantly increased when further supplemented with 4 mg/kg VA. Slc2a2 mRNA abundance was significantly increased in the 2 mg/kg VA group (linear, P = 0.024). Moreover, the budding rates, buddings number per organoid, and Chromogranin A and Muc2 expression of piglet intestinal organoids were significantly reduced (P < 0.05) by VA and its metabolites (retinoic acid). Compared with the control group, the expression of Spp1 and Trop2 increased. These results indicated that VA may increase the stemness of intestinal stem cell in vitro. This study suggested that VA could affect growth performance and intestinal function by regulating intestinal stem cells in the jejunum of weaned piglets.

文章來源：豬營養(yǎng)國際論壇
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